Psh65 plasmid
WebApr 30, 2016 · The yeast Saccharomyces cerevisiae is an important eukaryotic workhorse in traditional and modern biotechnology. At present, only a few S. cerevisiae strains have been extensively used as engineering hosts. Recently, an astonishing genotypic and phenotypic diversity of S. cerevisiae was disclosed in natural populations. WebNational Center for Biotechnology Information
Psh65 plasmid
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WebSelection Ampicillin, delta-ura3 Source Yeast, Escherichia coli Species Bacterial Q&A Customer Reviews Related Products For research use only. Not intended for any clinical use. Quick Inquiry Name Service & Products Interested * pSH65 (OVT2571) RESET USA SUITE 115, 17 Ramsey Road, Shirley, NY 11967, USA Germany Industriepark Höchst, Gebäude … WebApr 10, 2012 · To remove the hygromycin B resistance marker gene hphNT1, IMI058 and IMI059 were transformed with pSH65 and—after marker removal via the Cre/loxP system (Gueldener et al. 2002) and curing of the pSH65 plasmid—designated IMI062 …
WebThe Kan (r) marker was rescued by transforming plasmid pSH65 into positive transformants and inducing expression of Cre recombinase in galactose-containing medium. Lastly, the … WebOct 25, 2024 · To obtain the engineered Saccharomyces cerevisiae for high-yield endoglucanase, endoglucanase gene was cloned from Clostridium cellulovorans, with a total length of 1 996 bp, encoding 440 amino...
WebpSH65 Description Plasmid type: yeast Gene knockout vector Copy Number: High copy Promoter: GAL Cloning Method: Multiple cloning sites,restriction endonuclease Size: 8138 … http://euroscarf.de/plasmid_details.php?accno=P30122
WebStudy population. One hundred and ten consecutive patients undergoing allograft transplantation at Nantes University Hospital and at risk of CMV active infection were …
WebConstruction of plasmid pCU563 carrying a heterologous selection-marker gene flanked by loxP sites. A DNA fragment containing 34-bp loxP, the 0.45-kb PGKpromoter ( pr), and the … prayer for pastors 2021WebThe above-mentioned integrated plasmid contains components: pMB1 replicon, ampicillin resistance encoding gene, G418 resistance selection marker KanMX, δ1 fragment, δ2 fragment, GPD promoter, ADH1 terminator, TEF1 promoter and CYC1 terminator. The integrated plasmid is referred to as plasmid pND04 for short. scioto ridge vacation bible schoolWebJul 1, 2013 · Loss of pSH65 was achieved by growing cells in liquid YPD medium at 30 °C for 2 days. 2.3. Determination of extracellular inulinase activity Recombinants were grown in 50 mL of YPI medium (2% peptone, 1% yeast extract and 2% inulin [w/v], pH 5.5) at 30 °C with shaking at 200 rpm. scioto river water level piketon ohioWebpSH65: Cre-loxP plasmid used to remove the kanMX gene from the LycoYeast strain. Encodes for the Cre-recombinase under the control of the galactose-inducible promoter … scioto motel warehouse waverly ohioWebSep 13, 2024 · Transform pMM296 (pSH65 Carter and Delneri, 2010) into the strain of interest, plate on YPD, and replica plate onto YPD + 10 μg/mL Phleomycin the next day. The manufacturer's instructions are found here: Invivogen Product Information, Phleomycin. Inoculate Phleomycin-resistant colonies in YP+raffinose and let it grow overnight to … scioto river kayaking columbus ohioWebThe present invention discloses a strain of bacteria producing DHA and/or EPA, six gene fragments in the bacterial genome, and uses thereof. The strain is Schizoochytrium limacinum HS01, which has the accession number of CGMCC No. 13746 at China General Microbiological Culture Collection Center. The six gene fragments are composed of gene … scioto river water level at circlevilleWebApr 14, 2024 · In addition, using the electrotransformation to remove the selected marker KanMX [ 32 ], the pSH65 plasmid was transformed into haploid yeast strains with LEU1 and PDC5 deletions, and finally, the recombinant yeast strains XFa7-P/XFα6-P and XFa7-L/XFα6-L were obtained. scioto reserve homes for sale