Bodipy fl pepstatin a
WebApr 12, 2024 · To assess autolysosome formation and function in the same type of modified cells, the fluorescent probe BODIPY FL pepstatin A is used to monitor protease activity and vesicle acidification through the inhibition of cathepsin D, a protease found in the lysosome that participates in the APP degradation (Chen et al., 2000; Boland et al., 2008 ... WebDescription. BODIPY™ FL pepstatin A, a green-fluorescent probe, which inhibits cathepsin D in vitro (IC50 approx. 50nM), targets cathepsin D within lysosomes of live …
Bodipy fl pepstatin a
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WebThe residual rSap9, but not rSap10, activity could be further reduced at higher pepstatin A concentrations, with 40% and 27% residual activity at pH 6 when applying 50 and 100 M pepstatin A ... WebIn vitro, BODIPY FL-pepstatin A inhibits cathepsin D activity with an IC 50 of 10 nM. The nature of its binding to cathepsin D was further characterized using a fluorescence polarization measurement. Results showed that BODIPY FL-pepstatin A selectively binds to cathepsin D at pH 4.5.
WebThe BODIPY FL-pepstatin A is a potent inhibitor for cathepsin D. It inhibits the protease activity of cathepsin D with an IC 50 ~50 nM; 2. The binding of BODIPY FL-pepstatin A … WebMar 16, 2000 · In vitro, BODIPY FL–pepstatin A inhibits cathepsin D activity with an IC 50 of 10 nM. The nature of its binding to cathepsin D was further characterized using a …
WebFor lysosomal pH, Cathepsin D assay or Cathepsin L assay, 1 µM LysoSensor Green (Invitrogen, L7535), 1 µM BODIPY FL-pepstatin A (Invitrogen, P12271) or 20 μl Magic Red (ImmunoChemistry Technologies, 941) were incubated with hepatocytes at 37°C for 30 min. For F-actin staining, 100 nM SiR-Actin (Cytoskeleton Inc, CY-SC001) were incubated in ... WebJan 9, 2024 · BODIPY-FL-pepstatin A for active cathepsin D staining was obtained from Thermo Fisher Scientific (P12271). The experiments were conducted in accordance with the protocol provided by the manufacturer. Briefly, the sciatic nerves from uninjured or injured mice were harvested, and then connective tissues were detached in PBS.
WebMar 16, 2000 · The BODIPY FL fluorophore is very photostable and pH-insensitive, two important properties for both microscopic studies and enzymatic assay [16]. The …
WebMar 16, 2000 · In vitro, BODIPY FL-pepstatin A inhibits cathepsin D activity with an IC50 of 10 nM. The nature of its binding to cathepsin D was further characterized using a … pulse in the bibleWebApr 25, 2011 · For BODIPY FL pepstatin A staining, cells were labeled with 1 μM BODIPY FL pepstatin A for 30 min, and then the probe-containing medium was replaced with fresh medium. Electron Microscopy. Cells were fixed in 3% glutaraldehyde in 0.1 M Mops buffer (pH 7.0) for 8 h at room temperature, then 3% glutaraldehyde/1% paraformaldehyde in … pulse interval modulation codesWebOct 28, 2013 · Probing the cathepsin D using a BODIPY FL-pepstatin A: applications in fluorescence polarization and microscopy J. Biochem. Biophys. Methods (2000) M. Koike et al. Involvement of two different cell death pathways in retinal atrophy of cathepsin D-deficient mice Mol. Cell. Neurosci. (2003) N. Zaidi et al. pulse in the eyelidWeb开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 sebaceous cyst on catsWebDec 28, 2024 · Lipid peroxidation was measured by staining using C11 BODIPY, and immunofluorescence images were taken using a laser-scanning confocal microscope (LSCM). ... glycerol (2%). EDTA (1 mM), DTT (1 mM), aprotinin (1 μg/ml), leupeptin (1 μg/ml), pepstatin (1 μg/ml), PMSF (1 ... levels was measured by counting the filipin … sebaceous cyst on ball sackWebOther suitable methodologies rely on the use of fluorescently labelled inhibitors of lysosomal hydrolases such as Bodipy FL-Pepstatin A (Invitrogen, P12271), a … sebaceous cyst on back inflamedWebNeuromics primary human neurons Primary Human Neurons, supplied by Neuromics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more pulse in the head